Datura stramonium agglutinin: Cloning, molecular characterization and recombinant production in Arabidopsis thaliana

Datura stramonium agglutinin: cloning, molecular characterization and recombinant production in Arabidopsis thaliana.

Datura stramonium seeds contain at least three chitin-binding isolectins [termed Datura stramonium agglutinin (DSA)] as homo- or heterodimers of A and B subunits. We isolated a cDNA encoding isolectin B (DSA-B) from an immature fruit cDNA library; this contained an open reading frame encoding 279 deduced amino acids, which was confirmed by partial sequencing of the native DSA-B peptide. The seq...

Cloning and Characterization of an Arabidopsis thaliana

cDNA and genomic clones encoding DNA topoisomerase I were isolated from Arabidopsis thaliana Xgtl1 and XFix libraries by low stringency hybridization with a Saccharomyces cerevisiae TOPi probe. The cDNA clones include a 2748-base pair open reading frame predicting an amino acid sequence that is highly homologous to sequences encoded by TOP1 from yeast and human sources. The sequence of the upst...

Datura stramonium poisoning in children.

Although substance abuse is fairly common among adolescents, poisoning from Datura stramonium (a broadleaf annual erect herb with spine-covered seed capsule) is uncommon in children and has not been reported in our locality. We present the case of two children admitted at the Children Emergency Room of a teaching hospital following ingestion of extract of Datura stramonium. They developed neuro...

Poisoning by Datura (Stramonium)— Recovery

partaken of a quantity of datura leaves, prepared in the form of curry, with rice, which he afterwards acknowledged to have voluntarily taken. On admission, he lay perfectly unconscious, with severe spasmodic muscular twitchings and stertorous breathing. Skin cool; pulse about 108?, feeble. Pupils dilated and insensible to the effects of light. The stomach pump was immediately introduced, but d...

Jimson weed (Datura stramonium) poisoning in childhood.